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Test ID BLOD1482 Activated Protein C Reflex to Factor V Leiden

Container Type

Sodium Citrate and EDTA

Specimen Type/Requirements

Sodium Citrate Plasma – Double Spun Platelet Poor  and EDTA Whole Blood
(See Coagulation Testing and Platelet Poor Plasma Preparation)

Specimen Volume

2.0 ml Sodium Citrate plasma and 2.0 mL EDTA whole blood.

Minimum Volume

0.7 mL Sodium Citrate plasma
2.0 mL EDTA whole blood


Citrated Plasma:

Room Temp - 4 hours

Refrigerated - NA

Frozen - 1 month


EDTA Whole Blood

Room Temp - NA

Refrigerated - 7 days

Frozen - 12 months

Interfering Substances

Hemolysis: Yes if gross
Lipemia: NA

Transport Temperature



EDTA sample should not be used for any other testing in order to prevent contamination.

Performed Test Frequency

Monday and Thursday


APTT-Based Assay (Clot-Based)

Performing Lab

Sanford Sioux Falls