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HelpTest ID MICR0037 Culture Bacterial, Stool with Shiga-Like Toxin
Specimen Type/Requirements
Orange Cap Para-Pak C&S (Cary Blair Transport Media) - Stool (Refer to Stool Collection Patient Instruction link below)
Also acceptable:
Green Cap Para-Pak Enteric Plus (Cary Blair Transport Media) - Stool (Refer to Stool Collection Patient Instruction link below)
Red Cap Enteric Transport Medium (ETM) - Stool (Refer to Stool Collection Patient Instruction link below)
Sterile Container - Stool (only stable 2 hours refrigerated)
Note: Stool that is not in transport media and is received greater than 2 hours after collection may be detrimental to the recovery of Shigella spp.
Rectal swabs should be placed in Cary Blair Media immediately.
Please reference links for patient instructions:
Specimen Volume
| Preferred Volume | 2.0 grams |
|---|
Performed Test Frequency
Monday through Friday. Positives are reported the same day as set up. Negatives are “ENHANCED” and set up again the next day.
Methodology
Culture and Enzyme Immunoassay
Performing Lab
Sanford Laboratories Sioux Falls
Stability/Transport
Orange Cap Para-Pak C&S; Green Cap Para-Pak Enteric Plus (Cary Blair Transport Media) or Red Cap Enteric Transport Medium (ETM):
| Room Temperature | Not Acceptable | |
|---|---|---|
| Refrigerated | 24-72 hours* | Preferred for transport |
| Frozen | Not Acceptable |
*Refrigerated Cary Blair samples should be processed within 24 hours for best recovery of pathogens.
Steile Container:
| Room Temperature | Not Acceptable | |
|---|---|---|
| Refrigerated | 2 hours | Preferred for transport |
| Frozen | Not Acceptable |
Additional Information
Test includes: Stool Culture (Includes Salmonella/Shigella, Campylobacter, E. coli O157, Aeromonas, Vibrio and Plesiomonas) and Rapid Screening for Shiga-Like Toxin 1 And 2 Antigens.
Identification, typing, and/or sensitivity testing will be performed if indicated at an additional fee (per organism).
If stool is positive for E. coli O157, then H7 typing will be performed.
Follow-up cultures on previous positive patients should be ordered as a screen for a specific pathogen (MICR0002).