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Test ID MICR0019 Culture, Acid Fast Bacillus with Stain

AKA

Acid Fast Bacilli Culture, TB Culture

Specimen Type/Requirements

See specific source for specimen collection.

  • BLOOD: Refer to BLOD1118
  • GASTRIC CONTENTS:
    • Following an overnight fast, except water, a disposable plastic tube is passed through the nares into the stomach. A 10 mL syringe is then attached to the tube and the gastric contents aspirated by suction. It is necessary to put fluid through the tube to withdraw gastric material. A specimen of 10-15 mL is usually obtained with ease.
    • Gastric contents must be processed immediately after collection, sent to the Lab promptly.
    • Do not schedule this procedure prior to 7:00 a. m., after 5:00 p.m.or on weekends or holidays UNLESS SPECIAL ARRANGEMENTS ARE MADE WITH THE MICROBIOLOGY LABORATORY IN ADVANCE (Contact Microbiology by dialing: 701-417-7383).  Microbiology will notify the SMCF Lab when special testing requests have been made for patients from SMCF.
  • SPUTUM: Three consecutive first morning specimens.
  • TISSUE: Collect material using sterile technique. Place tissue in a sterile container.
  • STERILE FLUIDS: (pleural, thoracentesis, etc): Send 10 mL of fluid in a sterile container. DO NOT send all of the fluid collected.
  • URINE: Three consecutive first morning specimens.
    • Submit specimen in sterile container. Do not submit specimen on swab.

Specimen Volume

Refer to Body Fluid Collection Min-Max Volumes 12.80.L02 for preferred body fluid volumes.

Stability/Transport

Room Temperature 48 Hours Preferred Transport
Refrigerated 7 days  
Frozen N/A  

 

Performed Test Frequency

Monday-Sunday. Acid fast smears are not routinely stained on Sunday.

Methodology

Culture/Stain

Performing Lab

Sanford Laboratories Fargo-Microbiology

CPT

87116, 87206, 87015

Additional Information

Body Fluids: Concentration and Acid Fast Bacilli Stain by Fluorochrome will be performed when there is sufficient sample quantity available at an additional charge. Identification will be performed if indicated and an additional fee (per organism) may be applied. Susceptibility testing will only be performed upon request at an additional fee (per organism).