Test ID BLOD1382 Antibody to Extractable Nuclear Antigen Evaluation, Serum

Reporting Name

Ab to Extractable Nuclear Ag Eval,S

Useful For

Evaluating patients with clinical features suggestive of antinuclear antibody (ANA) associated connective tissue disease. May also be indicated in patients who test negative for ANA and have features of Sjogren syndrome and idiopathic inflammatory myopathies

Profile Information

Test ID	Reporting Name	Available Separately	Always Performed
SSA	SS-A/Ro Ab, IgG, S	Yes	Yes
SSB	SS-B/La Ab, IgG, S	Yes	Yes
SM	Sm Ab, IgG, S	Yes	Yes
RNP	RNP Ab, IgG, S	Yes	Yes
SCL70	Scl 70 Ab, IgG, S	Yes	Yes
JO1	Jo 1 Ab, IgG, S	Yes	Yes

Testing Algorithm

For more information see Connective Tissue Disease Cascade.

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Serum

Specimen Required

Collection Container/Tube:

Preferred: Red top

Acceptable: Serum gel

Submission Container/Tube: Plastic vial

Specimen Volume: 0.5 mL

Collection Instructions: Centrifuge and aliquot serum into a plastic vial.

Specimen Minimum Volume

0.35 mL

Specimen Stability Information

Specimen Type	Temperature	Time	Special Container
Serum	Refrigerated (preferred)	21 days
	Frozen	21 days

Special Instructions

Connective Tissue Disease Cascade

Reference Values

SS-A/Ro ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

SS-B/La ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Sm ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

RNP ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Scl 70 ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Jo 1 ANTIBODIES, IgG

<1.0 U (negative)

≥1.0 U (positive)

Reference values apply to all ages.

Day(s) Performed

Monday through Saturday

Test Classification

This test has been cleared, approved, or is exempt by the US Food and Drug Administration and is used per manufacturer's instructions. Performance characteristics were verified by Mayo Clinic in a manner consistent with CLIA requirements.

CPT Code Information

86235 x 6

Report Available

Same day/1 to 2 days

Reject Due To

Gross hemolysis	Reject
Gross lipemia	Reject
Gross icterus	OK
Heat-Treated	Reject

Method Name

Multiplex Flow Immunoassay

Secondary ID

89035

Sanford Laboratories - Bemidji Additional Information:

Sanford Interface Build Information

Result Code	Result Code Description
20334	SS-A/RO Antibodies Interpretation
20322	SS-A/RO Antibodies Numeric
20335	SS-B/LA Antibodies Interpretation
20323	SS-B/LA Antibodies Numeric
20340	SM Antibodies Interpretation
20328	SM Antibodies Numeric
20339	RNP Antibodies Interpretation
20327	RNP Anitbodies Numeric
20343	SCL-70 Antibodies Interpretation
20331	SCL-70 Antibodies Numeric
20342	JO-1 Antibodies Interpretation
20330	JO-1 Antibodies Numeric