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Test ID BLOD0119 Osmotic Fragility, Erythrocytes

Reporting Name

Osmotic Fragility, RBC

Useful For

Evaluation of suspected hereditary spherocytosis associated hemolytic anemia

 

Confirming or detecting mild spherocytosis

Profile Information

Test ID Reporting Name Available Separately Always Performed
FRAGO Osmotic Fragility No Yes
SCTRL Shipping Control Vial No Yes

Performing Laboratory

Mayo Clinic Laboratories in Rochester

Specimen Type

Control
Whole Blood EDTA


Advisory Information


 



Additional Testing Requirements


 



Shipping Instructions


Specimens must arrive within 72 hours of collection.



Necessary Information


Patient's age and sex are required.



Specimen Required


Both a whole blood EDTA specimen and a control specimen are required as temperature extremes can increase the fragility of the specimen and cause false-positive results.

 

Patient:

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Immediately refrigerate specimen after collection. Refrigerate at 0 to 4° C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.

2. Send specimen in original tube. Do not aliquot.

3. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.

4. Be sure specimen and control are stored and transported together at refrigerated temperature, carefully following proper handling and shipping instructions.

 

Normal Shipping Control:

Specimen Type: Whole blood

Container/Tube: Lavender top (EDTA)

Specimen Volume: 4 mL

Collection Instructions:

1. Draw a control specimen from a normal (healthy), unrelated, nonsmoking person at the same time as the patient.

2. Handwrite "normal control" clearly on the outermost label.

3. Immediately refrigerate specimen after collection. Refrigerate at 0 to 4° C. Do not freeze. Freezing causes sample lysis, and tests will not be performed on hemolyzed specimens.

4. Send specimen in original tube. Do not aliquot.

5. Rubber band patient specimen and control vial together. Control must accompany the patient sample at all times to ensure the reliability of testing results.


Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time
Control Refrigerated 72 hours
Whole Blood EDTA Refrigerated 72 hours

Reference Values

≥12 months:

0.50 g/dL NaCl (unincubated): 3-53% hemolysis

0.60 g/dL NaCl (incubated): 14-74% hemolysis

0.65 g/dL NaCl (incubated): 4-40% hemolysis

0.75 g/dL NaCl (incubated): 1-11% hemolysis

 

Reference values have not been established for patients who are <12 months of age.

Day(s) and Time(s) Performed

Monday through Saturday

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information

85557

Analytic Time

2 days

Reject Due To

Hemolysis

Mild reject; Gross reject

Lipemia

NA

Icterus

NA

Other

Clotted

Method Name

Osmotic Lysis

Forms

If not ordering electronically, complete, print, and send a Benign Hematology Test Request (T755) with the specimen.

Sanford Laboratories - Bemidji Additional Information:

Sanford Interface Build Information

Result Code Result Code Description
13662 Osmotic Fragility
13663 OS FR 0.50 G/DL NACL
13664 OS FR 0.60 G/DL NACL
13665 OS FR 0.65 G/DL NACL
13666 OS FR 0.75 G/DL NACL
13667 Osmotic Fragility Comment
13668 Shipping Control Vial