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Test ID NBLD0242 Viral Culture

Container Type

Universal Transport Media (UTM)
Sterile Container

Specimen Type/Requirements

Nasal Washings – in UTM
Stool – in UTM
Urine – in UTM
CSF – in sterile container
Amniotic Fluid – in sterile container
Throat – in UTM
Eye exudate – in UTM
Tissue biopsy – in UTM
Vesicular fluid – in UTM

Stability

Prior to innoculation – Room Temp – 4 hours

Viral innoculation of UTM - Refrigerated – 4 days
CSF/Amniotic Fluid stable 4 days refrigerated. Do NOT place in UTM.

Interfering Substances

Hemolysis: NA
Lipemia: NA

Transport Temperature

Refrigerated

Instructions

General directions for Universal transport media preparation as follows:
The inoculated swab should be placed in 3.0 mL vial of universal transport media immediately after collection.
Send media refrigerated.
Indicate Source.
Wooden swabs, swabs with calcium alginate or cotton tips are unacceptable.

 

For nasal washings, place 0.5-1.0 mL in 3.0 mL vial of universal transport media.


For Stools, place pea size amount in 3.0 mL vial of universal transport media.


For Urine, place 3.0 mL of urine in a 3.0 mL vial of universal transport media AND send an additional 2-4 mL of urine in sterile container.


For CSF or amniotic fluid, DO NOT put CSF or amniotic fluid in transport media- send straight.
Submit a minimum of 1.0 – 2.0 mL in sterile container.

 

Virus listing with sources (in alphabetical order):
Adenovirus: CSF, eye exudate, nasopharyngeal washing, stool, throat, or urine.


Coxsackie A & B: CSF, pericardial fluid, stool, throat, or vesicular fluid


Echovirus: CSF, pericardial fluid, stool, throat, or vesicular fluid


Enterovirus: CSF, pericardial fluid, stool, throat, or vesicular fluid


Herpes simplex: Brain, CSF, eye exudate, genital, throat swab, tissue biopsy, or vesicular fluid


Influenza A & B: Nasopharyngeal washing or throat swab


Parainfluenza 1, 2, 3, 4: Nasopharyngeal (NP) swab or washing, throat swab


Poliovirus: CSF, stool, or throat swab


Respiratory Syncytial Virus: Not recommended if specimen transport requires greater than 30 minutes to the laboratory. Refer to RSV by DFA

 

 

Performed Test Frequency

Monday through Sunday

Methodology

Culture

CPT

87252

Performing Lab

Sanford Sioux Falls

Additional Information

NOTE: Molecular Methods (e.g.PCR) are recommended for the following:
Herpes Simplex Virus – Order code NBLD0132
Varicella Zoster Virus – Order code NBLD0361
Enterovirus – Order code NBLD5038
Meningitits/Encephalitis Panel – Code NBLD0536 includes
Herpes Simplex Virus, Varicella Zoster Virus and Enterovirus

Analytical Time

Genital: 7 days

Other: 14 days