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Test ID BLOD1304 ADAMTS13 Activity and Inhibitor Profile

Reporting Name

ADAMTS13 Activity and Inhibitor Profile

Useful For

Assisting with the diagnosis of congenital or acquired thrombotic thrombocytopenic purpura

Profile Information

Test ID Reporting Name Available Separately Always Performed
ADMFX ADAMTS13 Activity Assay No Yes
ADMIN ADAMTS13 Interpretation No Yes

Reflex Tests

Test ID Reporting Name Available Separately Always Performed
ADMIS ADAMTS13 Inhibitor Screen No No
ADMBU ADAMTS13 Inhibitor Bethesda Titer No No

Testing Algorithm

Testing begins with ADAMTS13 activity assay to evaluate the percent activity. If the ADAMTS13 activity assay is <30%, an inhibitor screen will be performed to look for specific ADAMTS13 inhibition. If specific inhibition is apparent, the titer of the inhibitor will be determined.

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type

Plasma Na Cit


Specimen Required


See Coagulation Studies in Special Instructions.

 

Specimen Type: Platelet-poor plasma

Collection Container/Tube: Light-blue top (citrate)

Submission Container/Tube: Plastic vials

Specimen Volume: 2 mL in 2 plastic vials each containing 1 mL

Collection Instructions:

1. Specimen must be drawn prior to replacement therapy.

2. Spin down, remove plasma, and spin plasma again.

3. Freeze specimens immediately at ≤-40° C, if possible.

4. Send specimens in the same shipping container.

Additional Information:

1. Double-centrifuged specimen is critical for accurate results as platelet contamination may cause spurious results.

2. If priority specimen, mark request form, give reason, and request a call-back.

3. Each coagulation assay requested should have its own vial.


Specimen Minimum Volume

2 mL

Specimen Stability Information

Specimen Type Temperature Time
Plasma Na Cit Frozen 14 days

Reference Values

ADAMTS13 ACTIVITY ASSAY

≥70%

 

ADAMTS13 INHIBITOR SCREEN

Negative

 

ADAMTS13 BETHESDA TITER

<0.4 BU

Day(s) and Time(s) Performed

Monday through Sunday; Varies

Test Classification

See Individual Test IDs

CPT Code Information

85397-ADAMTS13 activity assay

85335-ADAMTS13 inhibitor screen assay (if appropriate)

85335-ADAMTS13 Bethesda titer (if appropriate)

LOINC Code Information

Test ID Test Order Name Order LOINC Value
ADM13 ADAMTS13 Activity and Inhibitor Profile In Process

 

Result ID Test Result Name Result LOINC Value
61211 ADAMTS13 Activity Assay 53622-7
34586 ADAMTS13 Interpretation 69049-5

Analytic Time

ADAMTS13 Activity Assay: 24 hours/ADAMTS13 Inhibitor Assay: 1-3 days/ADAMTS13 Bethesda Titer: 1-3 days

Reject Due To

Hemolysis

Mild OK; Gross reject

Lipemia

Mild OK; Gross reject

Icterus

Mild OK; Gross reject

Other

NA

 

Method Name

ADMFX: Fluorescence Resonance Energy Transfer (FRET)

ADMIS, ADMBU: Mixing Studies

Forms

1. Coagulation Patient Information (T675) in Special Instructions

2. If not ordering electronically, complete, print, and send a Coagulation Test Request Form (T753) with the specimen (http://www.mayomedicallaboratories.com/it-mmfiles/coagulation-test-request-form.pdf)