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Test ID BLOD0171 1,25-Dihydroxyvitamin D, Serum

Reporting Name

1,25-Dihydroxyvitamin D, S

Useful For

As a second-order test in the assessment of vitamin D status, especially in patients with renal disease


Investigation of some patients with clinical evidence of vitamin D deficiency (eg, vitamin D-dependent rickets due to hereditary deficiency of renal 1-alpha hydroxylase or end-organ resistance to 1,25-dihydroxyvitamin D)


Differential diagnosis of hypercalcemia

Performing Laboratory

Mayo Medical Laboratories in Rochester

Specimen Type


Advisory Information

The 25-hydroxyvitamin D test (25HDN / 25-Hydroxyvitamin D2 and D3, Serum) in serum the preferred initial test for assessing vitamin D status and most accurately reflects the body's vitamin D stores. In the presence of renal disease or hypercalcemia, testing of 1,25-dihydroxy vitamin D (DHVD) might be needed to adequately assess vitamin D status.

Specimen Required

Patient Preparation: Fasting (4-hour preferred but not required)


Preferred: Serum gel

Acceptable: Red top

Specimen Volume: At least 1.5 mL

Specimen Minimum Volume

0.7 mL

Specimen Stability Information

Specimen Type Temperature Time
Serum Refrigerated (preferred) 7 days
  Frozen  28 days
  Ambient  7 days

Reference Values


<16 years: 24-86 pg/mL

≥16 years: 18-64 pg/mL


<16 years: 24-86 pg/mL

≥16 years: 18-78 pg/mL

Day(s) and Time(s) Performed

Monday through Friday; 3 p.m.

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the U.S. Food and Drug Administration.

CPT Code Information


LOINC Code Information

Test ID Test Order Name Order LOINC Value
DHVD 1,25-Dihydroxyvitamin D, S 62290-2


Result ID Test Result Name Result LOINC Value
8822 1,25-Dihydroxyvitamin D, S 62290-2

Analytic Time

2 days

Reject Due To


Mild OK; Gross reject


Mild OK; Gross OK


Mild OK; Gross OK



Method Name

Extraction/Liquid Chromatography-Tandem Mass Spectrometry (LC-MS/MS)